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Free radicals are active molecules that are present every where and may be of internal or external origin. One of the most important destructive effects of free radicals is promoting lipid peroxidation resulting membrane destruction. This process causes the destruction of membrane and changing activities of intra membrane enzymics and other proteins and releasing radicals that is initially harmful to cells. In this study essential oil and extract of Matricuria chamonilla and pure essential oil (Azulone) are emphasised to be studied as antioxidants and free radical scavenger. Tershio Butyl Hydroperoxide (t-BIT) was used because of its capability to produce lipid peroxidation. Varied compounds were used in three concentrations. The produced malondialdehyde (MDA) was measured by the spectrophotometrie at 535 mm. Also, the enzymatic activities of LDH and AST were measured. Thenc enzymes are as markers for determining lipid peroxidation levels and decreasing their activity indicate the inhibitoty power of the treated compound. The highest level for inhibition of MDA production were respectively Matricaria chamomilla (essential oil) (58%)> azulene (35.4%) > Matricaria chamomilla (extract) (16%). There is a direct relationship between MDA level with enzymatic activities of LDH and AST. Results indicate the antioxidant effect of Matricaria chomomilla. Surely, it is required to wide spread in vivo study on these compounds to determine side effects and appropriate doses for the best efficacy and clinical usages.