Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
oleh: Thapakorn Jaroentomeechai, Jessica C. Stark, Aravind Natarajan, Cameron J. Glasscock, Laura E. Yates, Karen J. Hsu, Milan Mrksich, Michael C. Jewett, Matthew P. DeLisa
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-07-01 |
Deskripsi
The ability to produce homogeneous glycoproteins is expected to advance fundamental understanding in glycoscience, but current in vivo-based production systems have several limitations. Here, the authors develop an E. coli extract-based one-pot system for customized production of N-linked glycoproteins.