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Chyle from the thoracic duct of dogs fed cream or corn oil was fractionated into chylomicrons of different particle size: <140 mμ, 140–200 mμ, and >200 mμ. The composition of chylomicrons was studied before and after washing with saline solutions. In general the percentages of protein, phospholipid, cholesterol, and cholesterol ester were greater in the small chylomicrons than in the larger ones.Washing the chylomicrons did not appear to affect their composition. Chylomicrons collected at different times during fat feeding showed significant differences in phospholipid and cholesterol concentrations. Storage of lymph at 4° was accompanied by alterations in phospholipid and cholesterol concentrations of the chylomicrons. The composition of chylomicrons is consistent with the hypothesis that at some stage during their formation the nontriglyceride components are present on the surface of the oil droplet.