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Callus induction and regeneration of fennel from embryo explants were stabilized in the presence of cefotaxime antibiotic and different plant growth regulators (PGRs). The experiments were conducted under a factorial experiment, based on a completely randomized design (CRD). Genotypes; Fasa, Meshkinshar and Hajiabad were applied under different concentration of cefotaxime (0 and 100 mg l^-1), NAA (0 and 0.2 mg l^-1), IAA (0 and 0.4 mg l^-1) and BAP (0, 0.5 and 1mg l^-1). Regeneration, proliferations and root induction were taken placed on studied media, after 35 days without sub-culturing. The highest rate of proliferation with 200 shoots per explant was observed on B5 medium, containing100 mg l^-1 cefotaxime and 1.0 mg l^-1 BAP.  Callus induction and proliferations were observed in all media containing 100 mg l^-1 cefotaxime that can be related to auxin like activity of cefotaxime in fennel tissue culture.