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Abstract Objective: To determine the association between pseudoexfoliation and glaucoma. Study Design: Cross-sectional analytical study. Place and Duration of Study: Eye Outpatient department of Combined Military Hospital, Multan from Jan to Dec 2019. Methodology: A total of 170 patients reporting to the eye outpatient department were examined for the presence of Pseudoexfoliation and glaucoma based on raised intraocular pressure >21mm Hg, increased cup disc ratio and visual field defects. Therefore, a total of 340 eyes were examined. Results: Out of a total of 252 eyes with pseudoexfoliation, 40 (15.9%) had glaucoma. This frequency of glaucoma was significantly higher in eyes with pseudoexfoliation 87 (98.9%), in comparison to eyes without pseudoexfoliation 1 (1.1%); p<0.01). Conclusion: Pseudoexfoliation was found to be strongly associated with glaucoma.