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Closantel is an antiparasitic drug marketed in a racemic form with one chiral center. It is meaningful to develop a method for separating and analyzing the closantel enantiomers. In this work, two enantiomeric separation methods of closantel were explored by normal-phase high-performance liquid chromatography. The influences of the chiral stationary phase (CSP) structure, the mobile phase composition, the nature and proportion of different mobile phase modifiers (alcohols and acids), and the column temperature on the enantiomeric separation of closantel were investigated in detail. The two enantiomers were successfully separated on the novel CSP of isopropyl derivatives of cyclofructan 6 and <i>n</i>-hexane-isopropanol-trifluoroacetic acid (97:3:0.1, <i>v</i>/<i>v</i>/<i>v</i>) as a mobile phase with a resolution (<i>R</i>s) of about 2.48. The enantiomers were also well separated on the CSP of <i>tris</i>-carbamates of amylose with a higher <i>R</i>s (about 3.79) when a mixture of <i>n</i>-hexane-isopropanol-trifluoroacetic acid (55:45:0.1, <i>v</i>/<i>v</i>/<i>v</i>) was used as mobile phase. Thus, the proposed separation methods can facilitate molecular pharmacological and biological research on closantel and its enantiomers.