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This study aimed to investigate the performance of microbial fuel cell (MFC) for simultaneous bioremediation of slaughterhouse wastewater and sustainable power generation. For the first time, an integrated system of tubular type microbial fuel cell (MFC) was used in this study. The MFC consisted of three concentric Plexiglas tubes; the inner tube was the anaerobic anodic compartment, the mid tube was the aerobic biocathodic chamber, and the outer tube act as an aerobic bioreactor for extended nitrification process. The MFC system was connected to a complementary external anaerobic bioreactor for denitrification process. The microbial fuel cell was inoculated with freshly collected activated sludge and was continuously fueled with simulated slaughterhouse wastewater. Results revealed that the removal efficiency of the chemical oxygen demand (COD) was up to 99%, and the power generation was 165 mW/m². Also, results demonstrated that maximum removal of NO₃^- via the denitrification process in the final effluent was 94.7% when the initial concentration of NO₃^- in the effluent of the extended bioreactor was 15.2 mg/L. Approximately; complete recovery of nitrogen gas was obtained in the complementary external anaerobic bioreactor. These results indicated that MFC could be a promising approach for slaughterhouse wastewater bioremediation and renewable power generation.