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The study was envisaged to compare oestrual mucus spinbarkeit value and fertility in crossbred cattle during heat stress and at comfortable phase. A total of 24 apparently healthy cyclic crossbred cows in oestrus were selected for the study during the phase November – January (Group I), which was identified as comfortable phase. Another group of 20 animals in oestrus were also selected during March- May (Group II), which was identified as heat stress phase. Cervico- vaginal discharge during oestrus was collected and stretchability measured by spinbarkeit test. All the animals were inseminated 12 h after the onset of oestrus and pregnancy of non-returned animals was determined by rectal examination on day 45 post AI. Mean spinbarkeit values of conceived and non conceived animals in Group I were 16.56 ± 0.65 cm and 12.80 ± 0.50 cm respectively with overall mean of 14.20 ± 0.54 cm. The corresponding values of group II animals were 17.60 ± 1.43 cm, 16.53 ± 1.31 cm and 16.8 ± 1.03 cm. Significantly higher spinbarkeit values were obtained in conceived animals of the both the groups. Conception rates during comfortable phase and at heat stress were 37.5 and 25 per cent respectively.