Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients
oleh: Roman Wölfel, Janusz T. Paweska, Nadine Petersen, Antoinette A. Grobbelaar, Patricia A. Leman, Roger Hewson, Marie-Claude Georges-Courbot, Anna Papa, Stephan Günther, Sung Sup Park
| Format: | Article |
|---|---|
| Diterbitkan: | Centers for Disease Control and Prevention 2007-07-01 |
Deskripsi
We developed a real-time reverse transcription–-PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection.