Analysis of Protein Stability by the Cycloheximide Chase Assay
oleh: Shih-Han Kao, Wen-Lung Wang, Chi-Yuan Chen, Yih-Leong Chang, Yi-Ying Wu, Yi-Ting Wang, Shu-Ping Wang, Alexey Nesvizhskii, Yu-Ju Chen, Tse-Ming Hong, Pan-Chyr Yang
| Format: | Article |
|---|---|
| Diterbitkan: | Bio-protocol LLC 2015-01-01 |
Deskripsi
Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much popularity in cancer research. Here we present a full cycloheximide chase assay in our laboratory using a lung adenocarcinoma cell line, CL1-5, as a model.