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<p>Abstract</p> <p>Flow cytometry, DNA fragmentation, ion ratiomateric analysis and NMR peaks characterized drug chemosensitivity of antineoplastic drugs. Hypotheses were: 1. The chemosensitive effect of different cancer cell lines is characteristic; 2. DNA fragmentation, ion ratiometric analysis suggest apoptosis status of tumor cells.</p> <p>Methods</p> <p>PC-3 cell lines were compared with DU-145, LNCaP cell lines in culture for the [Na]_iand [Ca]_iion sensing dyes, cell death, NMR peaks and apoptosis staining for chemotherapeutic action of different drugs.</p> <p>Results</p> <p>DNA fragmentation, ratiometric ions and fluorescence endlabelling plots were characteristic for cell lines and drug response. ³¹P-²³Na NMR spectra showed characteristic high phospho-choline and sodium peaks.</p> <p>Conclusion</p> <p>Flow cytometry, DNA fragmentation, ion ratiometric methods and NMR peaks indicated apoptosis and offered in vivo drug monitoring method.</p>