Engineering subtilisin proteases that specifically degrade active RAS
oleh: Yingwei Chen, Eric A. Toth, Biao Ruan, Eun Jung Choi, Richard Simmerman, Yihong Chen, Yanan He, Ruixue Wang, Raquel Godoy-Ruiz, Harlan King, Gregory Custer, D. Travis Gallagher, David A. Rozak, Melani Solomon, Silvia Muro, David J. Weber, John Orban, Thomas R. Fuerst, Philip N. Bryan
| Format: | Article |
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| Diterbitkan: | Nature Portfolio 2021-03-01 |
Deskripsi
Chen et al. describe a rational design of subtilisin mutants that degrade active RAS by cleaving a conserved sequence in switch 2. They further modified the active site to be dependent on a cofactor to generate high target specificity. Proteases engineered to cleave this region degraded RAS in vitro and in cells with a promise of adaptability for other target proteins too.