Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
oleh: Christopher R. Cromwell, Keewon Sung, Jinho Park, Amanda R. Krysler, Juan Jovel, Seong Keun Kim, Basil P. Hubbard
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-04-01 |
Deskripsi
Minimizing off-target effects is an important concern for therapeutic applications of CRISPR-Cas9. Here, the authors show that incorporating bridged or locked nucleic acids into crRNA improves editing kinetics and reduces off-target cleavage.