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The effects of pyridoxine foliar application on protein content, lipid peroxidation, proline accumulation, and superoxide dismutase and catalase activities were studied in water stressed leaves of Zea mays L. S.C 704. The experiment was conducted in factorial with a randomized complete block design with three replications. The experiment was done with combination of water deficit stress at two stages of growth, and with three concentrations of pyridoxine (vitamin B6) (0, 75, 150 ppm). The activity of catalase increased, activity of superoxide dismutase reduced, protein content decreased but proline and malondialdehyde content increased under water stress. Our results also showed that vitamin B6 can not act as an antioxidant in plant at least through the foliar application method.