Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking
oleh: Kento Ojima, Kazuki Shiraiwa, Kyohei Soga, Tomohiro Doura, Mikiko Takato, Kazuhiro Komatsu, Michisuke Yuzaki, Itaru Hamachi, Shigeki Kiyonaka
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-02-01 |
Deskripsi
The analysis of AMPA-type glutamate receptor (AMPAR) trafficking is essential for understanding molecular mechanisms of learning and memory, but the analytical tools are currently limited. Here, the authors report a method that combines affinity-based receptor labeling and bioorthogonal click chemistry to quantify AMPAR distribution and trafficking under physiological conditions.