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<i>Enterococcus</i> spp. are normal intestinal tract microflorae found in poultry. However, the last decades have shown that several species, e.g., <i>Enterococcus cecorum</i>, have become emerging pathogens in broilers and may cause numerous losses in flocks. In this study, two combinations (H1 and H2) of menthol, 1,8-cineol, linalool, methyl salicylate, γ-terpinene, p-cymene, <i>trans</i>-anethole, terpinen-4-ol and thymol were used in an in vitro model, analyzing its effectiveness against the strains <i>E. cecorum</i>, <i>E. faecalis</i>, <i>E. faecium</i>, <i>E. hirae</i> and <i>E. gallinarum</i> isolated from broiler chickens from industrial farms. To identify the isolated strains classical microbiological methods and VITEK 2 GP cards were used. Moreover for <i>E. cecorum</i> a PCR test was used.. Antibiotic sensitivity (MIC) tests were performed for all the strains. For the composition H1, the effective dilution for <i>E. cecorum</i> and <i>E. hirae</i> strains was 1:512, and for <i>E. faecalis</i>, <i>E. faecium</i> and <i>E. gallinarum</i>, 1:1024. The second mixture (H2) showed very similar results with an effectiveness at 1:512 for <i>E. cecorum</i> and <i>E. hirae</i> and 1:1024 for <i>E. faecalis</i>, <i>E. faecium</i> and <i>E. gallinarum</i>. The presented results suggest that the proposed composition is effective against selected strains of <i>Enterococcus</i> in an in vitro model, and its effect is comparable to classical antibiotics used to treat this pathogen in poultry. This may suggest that this product may also be effective in vivo and provide effective support in the management of enterococcosis in broiler chickens.