The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
oleh: Javad Baharara, Elaheh Amini, Mohammad Amin Kerachian, Mozhgan Soltani
| Format: | Article |
|---|---|
| Diterbitkan: | Mashhad University of Medical Sciences 2014-08-01 |
Deskripsi
Objective(s):Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage. Materials and Methods: Isolated rBMMSc were grown in DMEM supplemented with 10% FBS. The cells were exposed to different concentration of extract. After 21 days, Alizarin red staining, alkaline phosphatase assay and RT-PCR were performed. The results were analyzed by ANOVA software and P value