Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system
oleh: Dasom Mun, Ji-Young Kang, Yumin Chun, Da-Seul Park, Hyoeun Kim, Nuri Yun, Seung-Hyun Lee, Boyoung Joung
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2022-12-01 |
Deskripsi
PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full pluripotency, normal karyotype and spontaneous differentiation capability. This cell line provides a suitable model for investigating the physiopathology of PITX2 mutation in atrial fibrillation.