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This study was evaluated to determine by thin layer chromatography of their residues to !be found in animal tissues of tetracycline group antibiotics, which were widely used in Veterinary field.<p> It was used IN HCL and methanol in extraction of samples and an Amber]ite XAD-2 resin colomn for clean-up. Slicaigel 60 G, cellulose and slicagel HF₂₅₄ were applied aş adsorbent. It was shown that cellulo'se was better result than others.<p> Their recovery were approximetaly determined as 62 %, adding the tetracyclines at 0.5, 1.0 and ,1.5 ppm concentrations to the tissues (muscle, liver, kidney) which was not found the tetracyclines residues. The analyse sensitivity of this system was 1.0 ppm for liver and 0.5 ppm for muscle and kidney.<p> It was concluded that this method would be able to be used for determination of tetracyclines residues in animal tissues as routine.