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Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum
oleh: Xihui Xie, Xihui Xie, Hong Du, Hong Du, Jichen Chen, Jichen Chen, Muhammad Aslam, Muhammad Aslam, Muhammad Aslam, Wanna Wang, Wanna Wang, Weizhou Chen, Weizhou Chen, Ping Li, Hua Du, Hua Du, Xiaojuan Liu, Xiaojuan Liu
Format: | Article |
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Diterbitkan: | Frontiers Media S.A. 2021-12-01 |
Deskripsi
N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae. Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum. In total, 863 different N-glycopeptides corresponding to 639 N-glycoproteins were identified from P. tricornutum. These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum. Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway. Subsequently, 69 N-glycans corresponding to 59 N-glycoproteins were identified and classified into high mannose and hybrid type N-glycans. High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue. Hybrid type N-glycan harbored Man-4 with a terminal GlcNAc residue. The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae. The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.