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<p>Abstract</p> <p>This paper presents the fabrication and characterization of zinc oxide micro-flowers and their antibacterial activity. The micro-flowers of zinc oxide composed of hexagonal nanorods have been prepared via solution process using precursor zinc acetate di-hydrate and sodium hydroxide in 3 h of refluxing time at ~90&#176;C. The antibacterial activities of grown micro-flowers were investigated against four pathogenic bacteria namely <it>S. aureus, E. coli, S. typhimurium and K. pneumoniae</it> by taking five different concentrations (5&#8211;45 &#956;g/ml) of ZnO micro-flowers (ZnO-MFs). Our investigation reveals that at lowest concentration of ZnO-MFs solution inhibiting the growth of microbial strain which was found to be 5 &#956;g/ml for all the tested pathogens. Additionally, on the basis of morphological and chemical observations, a chemical reaction mechanism of ZnO-MFs composed of hexagonal nanorods was also proposed.</p>