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Bioremediation  based  on  microbial  technologies  has  been  extensively  used  for  treating  coloured textile wastewater. In this research, the  potential application of the indigenous and exogenous bacterial cultures, found as biofilm were investigated for colour removal.  Initial study in the  treatment of the textile  wastewater showed that the mechanism involved in decolourisation was degradation, which was carried out via molecular technique involving amplification of the DNA sequence responsible for decolourisation using pure dye; Orange. In laboratory scale experiment, the  bacteria were  grown as  mixed culture  in suspension  and biofilm using shake flasks technique. Their abilities to decolourise textile wastewater were studied under semianaerobic conditions. Generally, bacteria in the form of biofilms were found to remove colour at faster  rates compared  to that  of suspended cells. Evidence of biofilm formation during decolourisation of textile wastewater was also examined using SEM.