Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product
oleh: Lucia F. Zacchi, Dinora Roche-Recinos, Cassandra L. Pegg, Toan K. Phung, Mark Napoli, Campbell Aitken, Vanessa Sandford, Stephen M. Mahler, Yih Yean Lee, Benjamin L. Schulz, Christopher B. Howard
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-03-01 |
Deskripsi
Zacchi et al. attempt to bridge the gap between understanding the impact of process variables and determination of critical post-translational modifications on a highly complex glycoprotein, factor IX. They implement an LC-MS/MS approach based on data independent acquisition which allows measurement of all detectable peptides, even with heterogeneous modifications.