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The prohibition of antibiotics has led to extensive research and use of phytogenic feed additives. James Barrie Kirkpatrick described four subspecies of eucalyptus (family Myrtaceae), including <i>Eucalýptus globulus</i>, in 1974. The maximum concentrations of quercetin-3D-glycoside (1703.30 g/mL), astragalin (1737.82 g/mL), chlorogenic acid (342.14 g/mL), catechin (282.54 g/mL), rosmarinic acid (36.39 g/mL), and 3,4-dihydroxybenzoic acid (27.55 g/mL) were found in samples of ultrasonic extraction with ethyl alcohol (extraction module 1:5, temperature of 32 °C, an ultrasonic exposure time of 25 min). Antimicrobial activity was observed in all studied samples after 12 h of incubation (against gram-positive (<i>Bacillus subtilis)</i> and gram-negative (<i>Pseudomonas aeruginosa)</i> bacteria, as well as representatives of yeast fungi (<i>Candida albicans</i>)); a more pronounced antimicrobial effect (lysis zone) was observed after ultrasonic processing of extracts for 20 and 25 min. <i>Bacillus subtilis, Pseudomonas aeruginosa</i>, and <i>Candida albicans</i> had lysis areas of 10.0 mm (20 min extraction with ultrasonic treatment), 13.0 mm (20 min extraction without ultrasonic treatment), and 15.5 mm (25 min extraction with ultrasonic treatment), respectively. <i>E. globulus</i> was demonstrated to be a source of biologically active phenolic compounds with antibacterial and fungicidal activity. More research on the use of <i>E. globulus</i> in feed additives is required.