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Objective To investigate the effects of exosome secreted by macrophage J774A.1 on the proliferation and migration of mouse colorectal cancer cell lines MC38 and CT26 and potential mechanism. Methods The phenotype of J774A.1 cells was identified by flow cytometry.The morphological characteristics, size and surface markers of the exosome of J774A.1 cells were detected by transmission electronic microscopy, nanoparticle tracking analysis(NTA) and Western blot.The uptake of exosome by MC38 and CT26 cells was observed by fluorescence microscopy. Proliferation of MC38 and CT26 was detected by CCK8 assay. Migration ability of MC38 and CT26 was detected by wound healing assay. The expression of phosphorylated proteins p-MEK1/2 and p-ERK1/2 in proliferation-related pathways was detected by Western blot. Results J774A.1 cells are M2 macrophages expressing the cell surface marker molecules CD68 and CD206. J774A.1 exosomes can be taken up by colorectal cancer cells MC38 and CT26, and can promote their proliferation(P＜0.05), enhance their migration and activate MEK-ERK signaling pathway. Conclusions Macrophage J774A.1 exosomes can promote the proliferation and migration of colorectal cancer cell lines MC38 and CT26, which is potentially attributed to the increased phosphorylation of MEK-ERK signaling pathway.