Find in Library

The antibacterial and anti-biofilm effects of <i>Hypericum perforatum</i> oil against the common pathogens of periodontitis (<i>Escherichia coli</i>, <i>Streptococcus mutans</i>, <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Porphyromonas gingivalis</i>) was investigated. Disk diffusion (DD), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) approaches were applied to test the antimicrobial effects. In order to determine the anti-biofilm effects, the amount of bacterial biofilm formation was assessed using the microtiter plate technique. The anti-biofilm effects were then confirmed by determining the minimum biofilm inhibitor concentration (MBIC). The MIC, MBC, MBIC, and DD values were 64, 256, 512 μg/mL, and 14 mm for <i>Staphylococcus aureus</i>; 128, 256, 512 μg/mL, and 16 mm for <i>Streptococcus mutans</i>; 256, 512, 256 μg/mL, and 20 mm for <i>Escherichia coli</i>; 32, 128, 512 µg/mL, and 16 mm for <i>Enterococcus faecalis</i>; and 64, 128, 256 µg/mL, and 15 mm for <i>Porphyromonas gingivalis</i>, respectively. According to our results, <i>Hypericum perforatum</i> oil has antibacterial and anti-biofilm properties against the common bacteria associated with periodontitis.