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SNARE protein is an essential factor driving vesicle fusion in eukaryotes. Several SNAREs have been shown to play a crucial role in protecting against powdery mildew and other pathogens. In our previous study, we identified SNARE family members and analyzed their expression pattern in response to powdery mildew infection. Based on quantitative expression and RNA-seq results, we focused on <i>TaSYP137</i>/<i>TaVAMP723</i> and hypothesized that they play an important role in the interaction between wheat and <i>Blumeria</i> graminis f. sp. <i>Tritici</i> (<i>Bgt</i>). In this study, we measured the expression patterns of <i>TaSYP132</i>/<i>TaVAMP723</i> genes in wheat post-infection with <i>Bgt</i> and found that the expression pattern of <i>TaSYP137</i>/<i>TaVAMP723</i> was opposite in resistant and susceptible wheat samples infected by <i>Bgt</i>. The overexpression of <i>TaSYP137</i>/<i>TaVAMP723</i> disrupted wheat’s defense against <i>Bgt</i> infection, while silencing these genes enhanced its resistance to <i>Bgt</i>. Subcellular localization studies revealed that <i>TaSYP137/TaVAMP723</i> are present in both the plasma membrane and nucleus. The interaction between <i>TaSYP137</i> and <i>TaVAMP723</i> was confirmed using the yeast two-hybrid (Y2H) system. This study offers novel insights into the involvement of SNARE proteins in the resistance of wheat against <i>Bgt</i>, thereby enhancing our comprehension of the role of the SNARE family in the pathways related to plant disease resistance.