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Plasmid shuttle vectors are a common tool used to study yeast physiology. The majority of yeast plasmids have been optimized for <i>Saccharomyces cerevisiae</i> lab strain compatibility, relying on auxotrophic complementation as their selective property. We sought to construct a series of plasmid shuttle vectors to extend functionality beyond strains with auxotrophic requirements, and test compatibility across a diverse panel of yeasts. We constructed 18 plasmids which were successfully maintained by yeasts from several genera. From a panel of 24 yeast strains, these plasmids were maintained by 18 yeasts, spanning 11 species within the genera <i>Lachancea</i>, <i>Metschnikowia</i>, <i>Pichia</i>, <i>Saccharomyces</i>, and <i>Torulaspora</i>. Additionally, an integrated gene expression reporter was assayed for functional compatibility with the 18 strains. Plasmid-derived gene expression was observed for 13 strains, spanning five species within the <i>Saccharomyces</i> genus, in addition to <i>Torulaspora delbrueckii</i>. These results indicate that this plasmid series is broadly useful for advancements and applications within academia, biotechnology, and the food and fermentation industries for research utilizing diverse <i>Saccharomyces</i> and non-<i>Saccharomyces</i> yeasts.