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CircRNAs are newly identified special endogenous RNA molecules that covalently close a loop by back-splicing with pre-mRNA. In the cytoplasm, circRNAs would act as molecular sponges to bind with specific miRNA to promote the expression of target genes. However, knowledge of circRNA functional alternation in skeletal myogenesis is still in its infancy. In this study, we identified a circRNA–miRNA–mRNA interaction network in which the axis may be implicated in the progression of chicken primary myoblasts’ (CPMs) myogenesis by multi-omics (i.e., circRNA-seq and ribo-seq). In total, 314 circRNA–miRNA–mRNA regulatory axes containing 66 circRNAs, 70 miRNAs, and 24 mRNAs that may be relevant to myogenesis were collected. With these, the <i>circPLXNA2</i>-<i>gga-miR-12207-5P</i>-<i>MDM4</i> axis aroused our research interest. The <i>circPLXNA2</i> is highly differentially expressed during differentiation versus proliferation. It was demonstrated that <i>circPLXNA2</i> inhibited the process of apoptosis while at the same time stimulating cell proliferation. Furthermore, we demonstrated that <i>circPLXNA2</i> could inhibit the repression of <i>gga-miR-12207-5p</i> to <i>MDM4</i> by directing binding to <i>gga-miR-12207-5p</i>, thereby restoring <i>MDM4</i> expression. In conclusion, <i>circPLXNA2</i> could function as a competing endogenous RNA (ceRNA) to recover the function of <i>MDM4</i> by directing binding to <i>gga-miR-12207-5p</i>, thereby regulating the myogenesis.