SGR-based Reporter to Assay Plant Transcription Factor-promoter Interactions

oleh: Shisong Ma, Savithramma Dinesh-Kumar

Format: Article
Diterbitkan: Bio-protocol LLC 2014-08-01

Deskripsi

We developed an in vivo method to assay plant transcription factor (TF)–promoter interactions using the transient expression system in Nicotiana benthamiana (N. benthamiana) plants. The system uses the Arabidopsis stay green (SGR) gene as a reporter. Induction of SGR expression in N. benthamiana causes chlorophyll degradation and causes leaves to turn yellow.