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Romania is a wine-producing country, with a rich and long tradition in the field of viticulture. Field evaluation of the land and grapevines from a phytosanitary point of view is essential for establishing the most effective control methods for grapevine diseases and pests. Grapevine trunk diseases (GTD) are currently considered one of the biggest threats to vineyard sustainability. In this study, GTD symptoms were investigated by direct field observations in vineyards from Blaj, Bujoru, Murfatlar and Miniș in 2020 throughout the vegetation period. In 2021 samples of the grapevines, showing signs of decline in the previous year were analyzed following traditional methods based on macroscopic examinations. A total of 69 samples were analyzed and symptoms of four GTDs were identified: Esca disease; Eutypa dieback; Phomopsis dieback and Petri disease. Out of these 55 % of the samples presented Esca disease symptoms. More than 80 % of the samples presented symptoms of 2 or more diseases at the same time.