Protein Translation Study – Label Protein with S35 Methionine in Cells
oleh: Salma Merchant, Isabelle Plo-Azevedo
| Format: | Article |
|---|---|
| Diterbitkan: | Bio-protocol LLC 2012-11-01 |
Deskripsi
To follow protein synthesis, cells should be incubated with radioactive amino acid such as [35S] methionine during mRNA translation. Then, the neosynthetized protein will be identified by an autoradiography after immunoprecipitation with a specific antibody and separation on a polyacrylamide denaturing gel.