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A series of human and animal diseases that are caused by <i>Salmonella</i> infections pose a serious threat to human health and huge economic losses to the livestock industry. We found antibiotic resistance (AR) genes in the genome of 133 strains of <i>S.</i> Indiana from a poultry production site in Shandong Province, China. <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Indiana strain MHYL had multidrug-resistance (MDR) genes on its genome. Southern blot analysis was used to locate genes on the genomic DNA. High-throughput sequencing technology was used to determine the gene sequence of the MHYL genome. Areas containing MDR genes were mapped based on the results of gene annotation. The AR genes <i>bla</i>_TEM, <i>strA</i>, <i>tetA</i>, and <i>aac</i>(6&#8242;)-<i>Ib-cr</i> were found on the MHYL genome. The resistance genes were located in two separate MDR regions, RR1 and RR2, containing type I integrons, and Tn<i>7</i> transposons and multiple IS<i>26</i> complex transposons with transposable functions. Portions of the MDR regions were determined to be highly homologous to the structure of plasmid pAKU_1 in <i>S. enterica</i> serovar Paratyphi A (accession number: AM412236), SGI11 in <i>S. enterica</i> serovar Typhimurium (accession number: KM023773), and plasmid pS414 in <i>S.</i> Indiana (accession No.: KC237285).