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The classification of carbapenemases can help guide therapy. The present study evaluated the performance of the CPO detection test, included in the BD Phoenix^™ NMIC-501 panel for the detection and classification of carbapenemases on the representative molecularly characterized strains collection from Mexico. Carbapenem non-susceptible isolates collected in Mexico were included. The clinical isolates (<i>n</i> = 484) comprised <i>Klebsiella pneumoniae</i> (<i>n</i> = 154), <i>Escherichia coli</i> (<i>n</i> = 150), and <i>P. aeruginosa</i> (<i>n</i> = 180). BD Phoenix CPO NMIC-504 and NMIC-501 panels were used for the identification of species, antimicrobial susceptibility tests, and detection of CPOs. For the detection of carbapenemase-encoding genes, <i>E. coli</i> and <i>K. pneumoniae</i> were evaluated using PCR assays for <i>bla</i>_NDM-1, <i>bla</i>_KPC, <i>bla</i>_VIM, <i>bla</i>_IMP, and <i>bla</i>_OXA-48-like. For <i>P. aeruginosa</i>, <i>bla</i>VIM, <i>bla</i>IMP, and <i>bla</i>GES were detected using PCR. Regarding <i>E. coli</i>, the CPO panels had a sensitivity of 70% and specificity of 83.33% for the detection of a class B carbapenemase (<i>bla</i>_NDM in the molecular test). Regarding <i>K. pneumoniae</i>, the panels had a sensitivity of 75% and specificity of 100% for the detection of a class A carbapenemase (<i>bla</i>_KPC in the molecular test). The Phoenix NMIC-501 panels are reliable for detecting class B carbapenemases in <i>E. coli</i>. The carbapenemase classification in <i>K. pneumoniae</i> for class A carbapenemases has a high specificity and PPV; thus, a positive result is of high value.