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Combining ability analysis helps in the evaluation of inbreds interms of their genetic value and in the selection of suitableparents for hybridization. It is also useful technique in identification of superior cross combinations. In the present study,the general combining ability of the 19 parents and specific combining ability of 84 hybrids resynthesised using thoseparents were estimated through combining ability analysis for yield, yield components and fibre quality parameters in cotton(Gossypium hirsutum) using a Line x Tester design. The estimated components of GCA and SCA variances showedpreponderance of non additive gene action for the characters studied. This showed the possibilities of improvement of thesetraits through heterosis breeding. Based on the gca effects the lines TCH 1702, TCH 1726, HSC – 1 – 521 were identified asgood combiners for most of the characters. These parents can be used in pedigree breeding for incorporation of desiredtraits. The hybrid combinations TCH 1705 x HSC – 1 – 521, NARASIMHA x HSC – 1 – 521, TCH 1726 x HSC – 2 – 133,H 97 x HSC – 1 – 521 recorded as the highest specific combiners. These hybrids could be exploited commercially.