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This is a very first attempt to compile a checklist of spiders from Gujarat.  It is a result of primary data as well as based on published literature.  The spider diversity of Gujarat consists of 415 species under 169 genera and 40 families.  Out of these, 29 genera and 17 families are endemic to Gujarat.  Overall, high spider diversity was recorded from zone III with 33 families, 132 genera and 278 species.  Whereas, the zone with the least spider records was zone VI with only 16 families, 39 genera and 70 species.  This is an under estimated number and many new records as well as new species are expected as a result of more systematic studies on spiders in this region.