Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei
oleh: Soumya P. Behera, Abhinav Dubey, Wan-Na Chen, Viviane S. De Paula, Meng Zhang, Nikolaos G. Sgourakis, Wolfgang Bermel, Gerhard Wagner, Paul W. Coote, Haribabu Arthanari
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-11-01 |
Deskripsi
The structure and dynamics of large proteins and complexes can be studied by methyl-NMR but resonance assignment is still challenging. Here, the authors present a NMR method that leverages optimal control pulse design to unambiguously distinguish between Leu and Val using a simple 2D HMQC experiment and they apply it to several proteins including Cas9, interleukin, and human translation initiation factor eIF4a.