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<strong><strong><em></em></strong></strong><p align="left"><strong>Background</strong>:<span style="font-size: small; font-family: Times New Roman;"> AmpC bta lactamases play a significant role in creating resistance to </span>third generation cephalosporins worldwide. They mostly express on chromosome of Enterobacteriaceae especially <em><span style="font-size: small; font-family: Times New Roman;"><em><span style="font-size: small; font-family: Times New Roman;">Escherichia coli </span></em></span></em><span style="font-size: small; font-family: Times New Roman;">and cause consequential problem in</span>clinical treatment and lead to failure in diagnosis and phenotypic test recommended byClinical and Laboratory Standards Institute.</p><p align="left"><strong>Methods</strong>:<span style="font-size: small; font-family: Times New Roman;"><span style="font-size: small; font-family: Times New Roman;">Totally 200 </span></span><em><span style="font-size: small; font-family: Times New Roman;"><em><span style="font-size: small; font-family: Times New Roman;">E. coli </span></em></span></em><span style="font-size: small; font-family: Times New Roman;">isolates from different hospitals of Tehran were </span>collected. The isolates were screened by disk diffusion method according to the CLSI guidelines. The profiles and prevalence surveys of AmpC (Dha, CITM, Mox and FOX-<span style="font-size: small; font-family: Times New Roman;" lang="JA"><span style="font-size: small; font-family: Times New Roman;" lang="JA">type) β</span></span><span style="font-size: small; font-family: Times New Roman;"><span style="font-size: small; font-family: Times New Roman;">-lactamase genes in clinical isolates of </span></span><em><span style="font-size: small; font-family: Times New Roman;"><em><span style="font-size: small; font-family: Times New Roman;">E. coli </span></em></span></em><span style="font-size: small; font-family: Times New Roman;">by phenotypic and </span>molecular methods. </p><p align="left"> <strong>Results</strong>:<span style="font-size: small; font-family: Times New Roman;"><span style="font-size: small; font-family: Times New Roman;">Out of 200 </span></span><em><span style="font-size: small; font-family: Times New Roman;"><em><span style="font-size: small; font-family: Times New Roman;">Ecoli </span></em></span></em><span style="font-size: small; font-family: Times New Roman;">isolated, 115 (89.8%) and 13 (10.2%) isolates were </span>identified as ESBL- and AmpC- beta-lactamase producers, respectively. Among mpC producers, 13 (100%) and 5 (38.5%) isolates was reported by PCR assay as bla-CITM and Dha respectively. Mox and FOX genes were not detected in any sample.</p><p align="left"><strong>Conclusions</strong>:<span style="font-size: small; font-family: Times New Roman;">Our results highlight the importance of using molecular detection </span>methods to identify β<span style="font-size: small; font-family: Times New Roman;"><span style="font-size: small; font-family: Times New Roman;">-lactamase-producer that have resistance to antibiotics</span></span></p><p><span style="font-size: xx-small; font-family: Times New Roman;">.</span></p><p> </p>