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Tungstate based nanomaterials have emerged as important class in transition metal oxide. In this study, Lead tungstate (PbWO _4 ) nanostructures with lamellar morphology were prepared by hydrothermal method. The synthesized materials were characterized by XRD, SEM, FTIR, DLS, BET and PL. Nitrogen adsorption-desorption measurements indicated that the surface area of the synthesized lamellar morphology was ∼86.225 m ^2 g ^−1 . The lamellar-like morphology showed enhanced peroxidase-like activity owing to the large surface area, higher substrate interaction and efficient electron transportation. The results indicated higher reaction velocity (V _max  = 13.56 × 10 ^−8 M s ^−1 ) and low Michaelis-Menten constant (km = 0.325 mM) value for nanostructures, providing evidence for higher affinity of novel structures towards the substrate and increased peroxidase-like activity. Finally, biocompatibility test was conducted by performing cytotoxicity experiments of PbWO _4 nanostructures on MTT assays.