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Human Machine Interfaces (HMI) principles are for the development of interfaces for assistance or support systems in physiotherapy or rehabilitation processes. One of the main problems is the degree of customization when applying some rehabilitation therapy or when adapting an assistance system to the individual characteristics of the users. To solve this inconvenience, it is proposed to implement a database of surface Electromyography (sEMG) of a channel in healthy individuals for pattern recognition through Neural Networks of contraction in the muscular region of the biceps brachii. Each movement is labeled using the One-Hot Encoding technique, which activates a state machine to control the position of an anthropomorphic manipulator robot and validate the response time of the designed HMI. Preliminary results show that the learning curve decreases when customizing the interface. The developed system uses muscle contraction to direct the position of the end effector of a virtual robot. The classification of Electromyography (EMG) signals is obtained to generate trajectories in real time by designing a test platform in LabVIEW.