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Introduction: Digit ratio is the ratio of the lengths between the 2nd Digit (index finger) and the 4th Digit (ring finger) which is measured from base of the proximal phalanx to the tip of distal phalanx. Digit ratio pattern has been associated with a variety of psychological sex-dimorphic variables, such as aggression, risk-taking behaviour and a possible higher risk for involving in Motor Vehicle Accident (MVA). Aim: To assess the prevalence and significance of Type 3 digit ratio in female motorcyclists involved in the MVA. Materials and Methods: This study was a comparative crosssectional study that included 194 female motorcyclists which were equally divided into MVA and non-MVA (control group). Radiograph of the right hand was used to calculate the digit ratio. The ratio were grouped into Type 1 (index>ring) common female pattern, Type 2 (index=ring) intermediate and Type 3 (index<ring) male pattern. Pearson's chi-square test was used to test the significance of association between the digit ratio pattern and the risk for MVA. Results: The result showed the MVA group was strongly associated with Type 3 pattern (53.6%) (p<0.05), while the nonMVA group was associated with Type 1 pattern (86.6%). Conclusion: The Type 3 digit ratio in female motorcyclists appears to be highly associated to involve in MVA.