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Background: Aflatoxins are fungal toxins which may be present in some foods and due to their negative health effects, represent a major concern for humans and food industries. In the present study, total aflatoxin contamination in products from eight feed materials production centers located in Qom City of Iran were evaluated by an ELISA technique in November 2012. Methods: A total of 40 feed samples were analyzed for total aflatoxin. The samples were collected randomly from eight feed materials production centers (C1-C8) located in Qom city. Samples were conditioned in sterile plastic container and kept at 4 ᵒC until analyses that were carried out in same day. Results: The total average of Aflatoxins concentration in samples were 1.83µg/kg. All samples demonstrated total aflatoxin levels lower than European Union standard and National Standard of Iran recommended limits. Conclusion: Considering the low values of aflatoxin contamination, maintaining vigilant preventive measures is recommended.These results do not preclude the need for continuing comprehensive studies for aflatoxin contamination.