CD8+ T cells mediate the athero-protective effect of immunization with an ApoB-100 peptide.

oleh: Kuang-Yuh Chyu, Xiaoning Zhao, Paul C Dimayuga, Jianchang Zhou, Xiaojun Li, Juliana Yano, Wai Man Lio, Lai Fan Chan, Jonathan Kirzner, Portia Trinidad, Bojan Cercek, Prediman K Shah

Format: Article
Diterbitkan: Public Library of Science (PLoS) 2012-01-01

Deskripsi

Immunization of hypercholesterolemic mice with selected apoB-100 peptide antigens reduces atherosclerosis but the precise immune mediators of athero-protection remain unclear. In this study we show that immunization of apoE (-/-) mice with p210, a 20 amino acid apoB-100 related peptide, reduced aortic atherosclerosis compared with PBS or adjuvant/carrier controls. Immunization with p210 activated CD8(+) T cells, reduced dendritic cells (DC) at the site of immunization and within the plaque with an associated reduction in plaque macrophage immunoreactivity. Adoptive transfer of CD8(+) T cells from p210 immunized mice recapitulated the athero-protective effect of p210 immunization in naïve, non-immunized mice. CD8(+) T cells from p210 immunized mice developed a preferentially higher cytolytic response against p210-loaded dendritic cells in vitro. Although p210 immunization profoundly modulated DCs and cellular immune responses, it did not alter the efficacy of subsequent T cell dependent or independent immune response to other irrelevant antigens. Our data define, for the first time, a role for CD8(+) T cells in mediating the athero-protective effects of apoB-100 related peptide immunization in apoE (-/-) mice.