Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets
oleh: Dragomir B. Krastev, Stephen J. Pettitt, James Campbell, Feifei Song, Barbara E. Tanos, Stoyno S. Stoynov, Alan Ashworth, Christopher J. Lord
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-05-01 |
Deskripsi
Poly ADP-ribosylation (PARylation) is a highly dynamic post-translation protein modification, but most methods only detect stable PARylation events. Here the authors develop a split-GFP-based sensor for PARylation detection in live cells and use it to identify a new centrosomal PARylation target.