Find in Library

Typhoid fever is a major global health problem and is the result of systemic infections caused by the human-adapted bacterial pathogen <i>Salmonella enterica</i> serovar Typhi (<i>S</i>. Typhi). The pathology underlying <i>S</i>. Typhi infections significantly differ from infections caused by broad host range serovars of the same species, which are a common cause of gastroenteritis. Accordingly, identifying <i>S</i>. Typhi genetic factors that impart functionality absent from broad host range serovars offers insights into its unique biology. Here, we used an in-silico approach to explore the function of an uncharacterized 14-gene <i>S</i>. Typhi genomic islet. Our results indicated that this islet was specific to the <i>S. enterica</i> species, where it was encoded by the Typhi and Paratyphi A serovars, but was generally absent from non-typhoidal serovars. Evidence was gathered using comparative genomics and sequence analysis tools, and indicated that this islet was comprised of Type VI secretion system (T6SS) and contact-dependent growth inhibition (CDI) genes, the majority of which appeared to encode orphan immunity proteins that protected against the activities of effectors and toxins absent from the <i>S</i>. Typhi genome. We herein propose that this islet represents an immune system that protects <i>S</i>. Typhi against competing bacteria within the human gut.