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For the last decades several studies for holding sperm have been done in various fowl species. Consequently different conditions were proposed, changing storage times, temperature, and adapting semen extenders for each situation. However outstanding development has been reached, each species and conditions demand special needs. Our previous studies were mainly conducted on the evaluation of the semen quality parameters in pheasants (Marzoni et al., 2000a,b; Marzoni et al., 2001; Chiarini et al., 2002; Marzoni et al., 2002), thus the present study focused on the use of three different semen extenders to preserve pheasant semen over a 24-hours period........