CARDON IMMUNO ASSAY: A SIMPLE AND RAPID SERODIAGNOSTIC TEST FOR CAPRINE TOXOPLASMOSIS

oleh: K. Syamala, K. Devada, K. Madhavan Pillai

Format: Article
Diterbitkan: Director of Academics and Research, Kerala Veterinary and Animal Sciences University 2007-01-01

Deskripsi

The efficacy of carbon immuno assay in the diagnosis of Toxoplasma gondii in goats was studied by subjecting 952 blood samples of goats maintained in the Government goat farms of Atfapady, Kommeri and KAU and of those brought to the slaughter houses of Thrissur and Ernakulam. The antigen required was prepared from the peritoneal exudates of T. gondii infected mice. Antigen coated slides were allowed to remain in contact with the serially diluted suspected sera at 37° C for 30 min followed by. staining with India-ink. Antibodies to T. gondii could be detected in 44.07 per cent of samples. It was indicated by more than 50% staining in sera with titres> 4. CIA is a simple and easy test and very cost effective in the diagnosis of toxoplasmosis in any species of animal