Single strand confirmation polymorphism (SSCP) detection in exon I of the <FONT FACE=Symbol>a</font>-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
oleh: Dinesh Kumar, Neelam Gupta, S.P.S. Ahlawat, R. Satyanarayana, Shyam Sunder, S.C. Gupta
| Format: | Article |
|---|---|
| Diterbitkan: | Sociedade Brasileira de Genética 2006-01-01 |
Deskripsi
The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect <FONT FACE=Symbol>a</font>-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the a-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These a-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection.