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We report the successful incorporation of pentacosadiynoic acid (PCDA) in poly(Ɛ-caprolactone) (PCL) by electro-spinning technique for sensor application. The UV-polymerization of the resulting electro-spun fibers mats (EFMs) produced polydiacetylenes (PDA) polymer exhibiting blue color. The PCL-PDA EFMs were characterized by Raman Spectroscopy, UV-Vis Spectroscopy and FE-SEM analysis. Sensor test results revealed that the treatment of the PCL-PDA EFMs with adulterated gasoline showed a blue to red color transitionrapidly. FE-SEM images demonstrated that the thinner or toluene used as an adulterant in the gasoline destroyed the PCL electro-spun fibers; which gave access to PDA polymer producing red color.