Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

oleh: W. Russ Algar, Igor L. Medintz, Guoliang Tao, Robert C. Triulzi, Samantha Spindel, Travis Jennings, Kim E. Sapsford

Format: Article
Diterbitkan: MDPI AG 2011-08-01

Deskripsi

The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took